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Projects. Life and Earth Sciences

Characterisation of the autoantigen epitope in Goodpasture syndrome using phage display technology

Lead Researcher:
Jesús Rodríguez Díaz 

Research Centre: 
Instituto de Agroquímica y Tecnología de Alimentos (IATA). CSIC. Valencia.  (Spain).

Synopsis:
Jesús RodríguezThis project aims to carry out a thorough characterisation of the epitope of the Goodpasture autoantigen (GP) by combining powerful techniques rarely used in our scientific environment. To achieve this goal recombinant forms of the a3 (IV) NC1 chain and the 3 non-collagenous domains a3(IV), a4(IV), a5(IV) will be produced together in the baculovirus system, thereby producing the GP antigen for the characterisation of circulating antibodies linked to the kidneys in GP patients. Fine characterisation of the epitope is made by combining mutagenesis aimed at a3(IV) NC1, using different phage display peptide libraries and the analysis of the binding kinetics of different antibodies to selected peptides and the different mutants produced by Surface Plasmon Resonance (SPR).  

SCIENTIFIC PRODUCTION  

2 articles published in Journals 
2 papers at national conferences
1 paper at international conferences 
________________________________________

Jesús Rodríguez Díaz  
From April 2001 to June 2005, I completed my doctoral thesis at the Department of Microbiology, University of Valencia and at the Division of Molecular Virology, University of Linköping (Sweden). The title of the thesis was "Study of immunogenicity and pathophysiological mechanisms of rotavirus NSP4 protein". The development of this thesis resulted in four publications in international journals as lead author [Rodriguez-Diaz, J., et al. (2003) Protein Expr Purif 31, 207-12, Rodriguez-Diaz, J., et al. (2004) J Virol Methods 121, 231-8, Rodriguez-Diaz, J., et al (2005) J Med Virol 77, 317-22, Rodriguez-Diaz, J., et al (2006) J Med Virol 78, 979-85.]

During this period I collaborated in the development of new vaccine strategies to confer protection against rotavirus infection by using DNA vaccines and the use of probiotic lactic acid bacteria to confer active and passive immunity (through participation in the Project Europe: "Probiotic strains with designed health properties" (Deprohealth)) and the use of yeast to confer active immunity [Garcia-Diaz, A., et al (2004) Vaccine 23, 489-98, Monedero, V., et al. (2004) Appl Environ Microbiol 70, 6936-9, Andres, I. et al. (2006) Biotechnol Bioeng 93, 89-98.].

I was also involved in calicivirus molecular epidemiology papers through participation in various European Projects: "Foodborne viruses in Europe: Rapid detection of transnational foodborne viral infections and elucidation of transmission routes through molecular tracing and development of a common database", "Providing tools to prevent emergence of enteric viruses (EVENT)" and "Prevention of Emerging (food-borne) enteric viral Infections: diagnosis, Viability testing, networking and epidemiology" (DIVINE-NET) [Buesa, J., et al. (2002) J Clin Microbiol 40, 2854-9.]

In my doctoral work at Linköping University in Sweden, I collaborated on the characterisation of the calicivirus cell receptor and the study of the genetic factors responsible for host susceptibility to norovirus infection and the development of evolutionary norovirus studies. [Larsson, MM, et al. (2006) J. Infect Dis 194, 1422-7; Rydell, G. E., et al. (2009) Glycobiology 19, 309-20; Carlsson, B., et al. (2009) J Gen Virol 90, 432-41.]

My first postdoctoral sojourn was at the Laboratory of Neurobiology of the Prince Philip Research Centre (CIPF). During this period I was involved in the study of the molecular basis for chronic hyperammonaemia syndrome (CHS) in the central nervous system (CNS) [Rodrigo, R., et al. (2007) J Neurochem 102, 51-64.]. Later I served as Scientific Director of the biotechnology company Biosensores S.L., participating in the development of an automated affinity biosensor based on magnetic particles.

In August 2007, I joined the Virus Biology Laboratory of the Microbiology and Cell Biology Centre at the Venezuelan Institute for Scientific Research (IVIC) as a contracted researcher . For a year I was the scientist responsible for two lines of research: environmental microbiology and molecular virology of caliciviruses. [Rodriguez-Diaz, J., et al. (2008) J Virol Methods 149, 240-5, Rodriguez-Diaz, J., et al (2005) J Med Virol 77, 317-22, Rodriguez-Diaz, J., et al (2006) J Med Virol 78, 979-85.] (2009) Appl Environ Microbiol 75, 387-94, Andres, I. et al.

From November 2008 to December 2009, I worked on the research staff at the Laboratory of Molecular Recognition of the CIPF. During this period I began my studies on the autoantigen in Goodpasture syndrome that led to the application for this project. Currently I work as a contracted researcher for the National Science Research Council (CSIC).


*All intellectual property rights belong to the author. Reproduction of all or part of the work without permission from the author is prohibited.

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