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Projects. Life and Earth Sciences

Application of genetic analyses for the creation of germplasm banks in teleostei

Lead Researcher:
Vanesa Robles Rodríguez 

Research Centre: 
Departamento de Biología Molecular e Instituto de Desarrollo Ganadero y Sanidad Animal (INDEGSAL). Universidad de León. (Spain).

Synopsis: 
Vanesa RoblesThis project aims to determine whether the study of gene expression in germ cells and testicular somatic cells would allow molecular diagnosis of sperm quality in male reproducers. To this end gene expression will be assessed using RT QPCR in fish sperm and testicular samples and parametric indicators of sperm quality, such as motility and viability, will be analysed; the latter being studied by flow cytometry. The aim is also to assess the genetic damage in the germline cells after cryopreservation. This project represents a qualitative leap forward in the analysis of semen quality in fish and in genetic damage assessment by incorporating new molecular biology technologies in the service of the aquaculture and conservation industries.


SCIENTIFIC PRODUCTION


4 articles published in Journals
1 paper at national conferences
4 papers at international conferences 
________________________________________

Vanesa Robles Rodríguez 
The researcher Vanesa Robles Rodriguez received her Ph.D. in 2004 from the University of Leon with a distinction cum laude and a European-wide special mention. Her thesis dealt with the cryopreservation of embryos and gametes in teleost fish and was awarded the Special Doctorate Prize by the University in 2006. Her first postdoctoral sojourn (2004-2007) was at the University of Algarve (Portugal). During this period her research focused on the study of antifreeze proteins (AFPs) to improve the cryopreservation of lines of special biotechnological interest as well as endangered species. From 2007 to 2009, she worked as a researcher at the Centre for Regenerative Medicine in Barcelona CMR(B). During these years, she carried out studies with primordial germ cells and embryonic stem cells of Danio rerio. She also carried out studies in regeneration using this fish as a model and used the ZFN protein applied to the production of knock-outs in this species. She has conducted numerous research sojourns in prestigious national and foreign institutions including: Marine Biological Laboratory (MBL) Woods Hole (USA), CBS, University of Alberta (Canada), Luton Institute of Research in the Applied Natural Sciences (United Kingdom) and the Oceanographic Centre at the University of Newfoundland (Canada). Her scientific production includes 40 publications (2 books, 8 book chapters - 2 of them currently in press, 19 scientific articles (SCI journals), 10 abstracts published in SCI journals and one review). Her book on Methods in Reproductive Aquaculture: marine and freshwater species (E. Cabrita, V. Robles and M.P. Herráez Eds.) includes information on endangered species, species of interest for aquaculture and species with high research potential, constituting a complete guide to sperm cryopreservation. She is currently on a Ramón y Cajal contract at the University of León where she carries out her teaching and research activity.


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