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Projects. Life and Earth Sciences

Study of the neuroprotector capacity of the epithelial cells of the choroid plexi as a potential regenerative therapy for Alzheimer's disease

Lead Researcher:
Eva María Carro Díaz

Research Centre:
Instituto de Investigación Hospital 12 de Octubre. Madrid.

Synopsis: 

Eva María Carro DíazThe search for alternative treatments which at least reduce the neurodegeneration characterising diseases such as Alzheimer's is an area of special scientific interest. The choroid plexi are involved in many of the basic aspects of neuronal functioning, including the secretion of a nutritious cocktail of polypeptides. They also play a role in repair processes following a lesion, such as occurs in ischaemia, or in Huntington's disease.

The aim of this study will be to determine the possible neuroprotective effects of the choroid plexus in Alzheimer's disease, by implanting epithelial cells from the choroid plexus in a murine model of Alzheimer's disease.

To this end the following aims are planned:

  • To determine the neuroprotective potential of the choroid plexus following implantation in the brain, in an animal model of amyloidosis.
  • To create a primary culture of human choroid plexus epithelial cells.

To date, work has been undertaken on the first objective. To this end, primary cultures of choroid plexus epithelial cells have been taken from C57BL6 strain rats 5 days after birth, according to the protocol described by Carro et al. (2002). After being kept in culture for one week, they are implanted in dual transgenic APP/Ps1 mice, which are transporters of mutations in the genes encoding for the amyloid precursor protein (APP) and preseniline 1 as the model for amyloidosis. For this, under anaesthesia with isoforane, and using stereotaxia, epithelial cells from the choroid plexus cultures are implanted bilaterally at the level of the hippocampus. Control animals are given the (saline) medium in which the cells had been re-suspended. Preliminary feasibility and cell implant survival tests are being conducted, with highly positive results one month after implantation.


Eva María Carro Díaz

Education:

  • 1991 Degree in Biology. Univ. of Santiago de Compostela. 1993.
  • 1992 Overall Degree Exam. Univ. of Santiago de Compostela. 1993.
  • 1993 Master's Degree in Ecological Management from the Ecological Research Institute. 420 teaching hours. Mark: Excellent. 1994.
  • 1994 Master's degree in Ecological Education from the Ecological Research Institute. 500 teaching hours. Mark: Excellent. 1997.
  • 1995 Master's degree in R&D+I Management and Direction in Health Sciences. King Charles III Research Institute. UNED. Univ. of Alcalá. 2011.

Current position:

  • Researcher (I3SNS Programme).
  • Head of the Neuroscience Group.
  • "12 de Octubre" Hospital Research Institute and CIBER for Neurodegenerative Diseases.

Publications:
More than 40 papers published in the past 10 years in international journals, including Nature Medicine, Biomaterials, Neurobiology of Aging, Neurobiology of Diseases, Acta Neuropathologica, Journal of Alzheimer Disease…

Research projects:
More than 10 research projects directed in the past 10 years and financed by public (King Charles III Health Institute) as well as private institutions.

Patents:

  • "The chemical composition of IGF‑I for the treatment and prevention of neurodegenerative diseases". Application nº P200200491 Date: 2005
  • "Animal model for neurodegenerative diseases: procedure for obtaining them and applications" Application nº P200401946 Date: 2006
  • "A non-human animal Alzheimer's disease model and uses thereof" Application nº PCT/EP2005/013022 Date: 2007
  • "The use of microparticles that include genetically modified cells in the treatment of neurodegenerative diseases" Application nº P200802217


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