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Characterisation of the autoantigen epitope in Goodpasture syndrome using phage display technology

15th National Research Competition in Life and Earth Sciences

Rare diseases

Senior Researcher : Jesús Rodríguez Díaz

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Research Centre or Institution : Instituto de Agroquímica y Tecnología deAlimentos(IATA). CSIC. Valencia. (Spain).

Abstract

This project aims to carry out a thorough characterisation of the epitope of the Goodpasture autoantigen (GP) by combining powerful techniques rarely used in our scientific environment. To achieve this goal recombinant forms of the a3 (IV) NC1 chain and the 3 non-collagenous domains a3(IV), a4(IV), a5(IV) will be produced together in the baculovirus system, thereby producing the GP antigen for the characterisation of circulating antibodies linked to the kidneys in GP patients. Fine characterisation of the epitope is made by combining mutagenesis aimed at a3(IV) NC1, using different phage display peptide libraries and the analysis of the binding kinetics of different antibodies to selected peptides and the different mutants produced by Surface Plasmon Resonance (SPR).

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