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Characterisation of the autoantigen epitope in Goodpasture syndrome using phage display technology
15th National Programme for the allocation of Research Grants for Life and Matter Sciences
Rare diseases
Senior Researcher : Jesús Rodríguez Díaz
Research Centre or Institution : Instituto de Agroquímica y Tecnología deAlimentos(IATA). CSIC. Valencia. (Spain).
Abstract
This project aims to carry out a thorough characterisation of the epitope of the Goodpasture autoantigen (GP) by combining powerful techniques rarely used in our scientific environment. To achieve this goal recombinant forms of the a3 (IV) NC1 chain and the 3 non-collagenous domains a3(IV), a4(IV), a5(IV) will be produced together in the baculovirus system, thereby producing the GP antigen for the characterisation of circulating antibodies linked to the kidneys in GP patients. Fine characterisation of the epitope is made by combining mutagenesis aimed at a3(IV) NC1, using different phage display peptide libraries and the analysis of the binding kinetics of different antibodies to selected peptides and the different mutants produced by Surface Plasmon Resonance (SPR).
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30
Jan
2020
International Symposium Understanding and reprogramming developmental visual disorders: from anophthalmia to cortical impairments Madrid, Thursday and Friday, 30 and 31 January 2020, 9:30 hours
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2024
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23
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