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Progression of different forms of multiple sclerosis related to the stem/progenitor cells potential
19th national competition for scientific and technical research
Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS): Molecular Etiology and Novel Treatments

Senior Researcher : Laura López Mascaraque
Research Centre or Institution : Instituto Cajal. CSIC. Madrid.
Abstract
The first objective of this project was to compare the mobilization of NG2-glial cells in multiplesclerosis (MS), using experimental autoimmune encephalomyelitis (EAE) and cuprizone (CPZ) models induced in C57BL/6 mice. To this end, we combined the StarTrack clonal analysis tool in both experimental MS models. Neural E14 progenitors were targeted using the StarTrack mix, to evaluate the clonal response of their derived NG2-glia progeny to brain damage at the peak of the disease in both models: EAE mice 15 days postinduction (dPI), and CPZ induced mice 35 dPI. Our results showed a heterogenic and clonal response of NG2-glia to the EAE induction in both cortex and the corpus callosum. This heterogeneous NG2-glia response was evident in both clonal proliferation and in their morphological change towards hypertrophy.
OA deeper analysis of NG2-glia morphology throughout the cortex was made in order to understand their morphological change due to inflammation, based on different morphometric parameters. We revealed that in physiological conditions there are at least three NG2-glia clusters: the first in both the corpus callosum (CC) and layer I, the second in the upper cortical layers, and the third one in lower cortical layers. Moreover, in response to EAE and CPZ, NG2-glia of the corpus callosum modified their morphology in a more significant way than in lower cortical layers cells.
The second objective was to analyze the cell response in postmortem human brains of the relapsing remitting (RRMS) and the primary progressive multiple sclerosis (PPMS). To address it, first we are performing a proteomic analysis of the cortex and the spinal cord (SC) in both experimental models. This will help us understand the pathophysiology of MS, to find proteins altered in the disease that may act as new targets for future therapeutic treatments. The expression of these altered proteins will be verified in the human tissue in order to characterize what type of cells express them in response to MS disease. For this, the human tissue was requested to the Netherlands Brain Bank, Amsterdam, and the immunohistochemical staining are currently made on control human tissue.
Scientific Production |
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Magazine Articles | 5 |
Communications at national conferences | 12 |
Communications at international conferences | 9 |
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4
Nov
2020
Conversaciones online desde la Fundación Ramón Areces La nueva visión de la ELA en el S.XXI. Bases moleculares. (Visión neuropatológica y genética) ONLINE, Wednesday, 4 November 2020, 19:00 hours
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26
Oct
2021
Jornada online Ensayos clínicos y búsqueda de nuevas dianas terapéuticas contra la ELA ONLINE desde: www.fundacionareces.tv/directo, Tuesday, 26 October 2021, 19:00 hours
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22
Jun
2022
International Symposium Cuidados paliativos globales: retos y expectativas futuras Madrid, Wednesday, 22 June 2022, 16:00 hours
- Investigation on hybrid molecules as powerful novel therapeutic approaches for Multiple Sclerosis and myelin- related rare diseases 2018 Senior Researcher : Fernando de Castro Soubriet Research Centre or Institution : Instituto Cajal. CSIC. Madrid.
- Significance of brain glucose hypometabolism and of altered insulin signal transduction in an experimental model of amiotrophic lateral esclerosis (ALE) 2018 Senior Researcher : Enrique Blázquez Fernández Research Centre or Institution : Universidad Complutense de Madrid
- Functional characterization and mechanisms of the signalling kinase MOK in neuroinfiammation associated to Amyotrophic Lateral Sclerosis (ALS) 2018 Senior Researcher : Cintia Roodveldt Research Centre or Institution : Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER)

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