Research projects

Plants can measure time and predict and adapt to coming environmental changes thanks to their endogenous circadian clock. The plant circadian clock accounts for one-third of Arabidopsis transcripts and therefore, it is involved in numerous biological and physiological processes. Understanding how the circadian signalling network regulates these processes and affects productivity is an important agronomic issue. Abiotic stresses including salinity, are a subject of increasing interest due to the significant impact on food yields in a scenario of continued reduction of arable land and water resources as well as climate change. Plant cells generate reactive oxygen and nitrogen species (ROS/RNS) involved in general metabolism that can be harmful for cellular components due to their chemistry, but they are also involved in cellular signalling to promote defense against environmental stress situations. Because ROS and RNS can be used as important secondary messengers during stress, it may be important for ROS and RNS homeostasis to be in tune with daily cycles to increase vegetative growth and enhance productivity of crops. This project will elucidate the connection between ROS/RNS homeostasis and the circadian signalling network. Thus, it will determine to what extend the Arabidopsis circadian network is involved in ROS homeostasis and whether is it is also involved in RNS homeostasis and in ROS/RNS responses to saline stress. Finally, it will aim to translate basic science into a possible biotechnological application by studying the almond tree circadian signaling network in order to identify genes that might enhance productivity. To date, we have optimized and carried out the determinations, in 24 h light-dark cycles, of the transcripts, enzimatic activities and metabolites levels that play a role in the ascorbate-glutathione cycle and in RNS homeostasis. Additionally, we have begun to study the possible connection between ROS/RNS-dependent posttranslational modifications with daily rhythms.