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Using genetic engineering to obtain strains of Clostridium cellulolyticum and Rhodobacter capsulatus that overproduce hydrogen

15th National Programme for the allocation of Research Grants for Life and Matter Sciences

Bioenergy

Senior Researcher : Alberto Sola Landa

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Research Centre or Institution : Instituto de Biotecnología de León (INBIOTEC).

Abstract

The micro-organisms capable of producing hydrogen naturally include Clostridium cellulolyticum and Rhodobacter capsulatus. The first is able to degrade cellulose by what is known as dark fermentation. As in all fermentative paths, the same amount of hydrogen is not always released, so the aim is to block the main genes on the alternative routes, while increasing the number of copies of key genes in the production. On the other hand, R. capsulatus releases hydrogen in conditions of light and lack of oxygen due to the activity of the nitrogenase enzyme. The goal is to eliminate the genes that down-regulate its activity as well as those consuming hydrogen. In addition, joint fermentations will be performed with both bacteria to further increase production.

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